Abstract
Development of the enzyme-containing nanocomposites provides an excellent opportunity for the development of the sensitive and effective analytical devices – biosensors. In the present work, we used the nanocomposites that contained two enzymes (glucose oxidase and hexokinase), polymers, and gold nanoparticles (GNPs). Such nanostructure was expected to increase electron transfer in the bioselective element of biosensor and to improve the enzyme stability during the immobilization process. An amperometric biosensor based on the bienzyme system has been developed. The biosensor is sensitive to adenosine-5′-triphosphate (ATP) and glucose. The enzymes were immobilized onto the surface of a platinum disk electrode that was used as amperometric transducer. Three different methods of immobilization were investigated: cross-linking of the enzymes in the presence of bovine serum albumin, entrapment in a photo-cross-linkable modified polyvinyl alcohol (PVA-SbQ) matrix, and entrapment of the enzymes in a PVA/polyethylenimine matrix. The best results during the ATP determination were obtained with PVA-SbQ, and this immobilization method was modified by addition of 18-nm GNPs to the reacting mixture. The ATP detection could be achieved in all cases except for PVA/polyethylenimine, the best sensitivity and linear range being achieved by co-immobilizing glucose oxidase/hexokinase into the photo-cross-linked PVA-SbQ/GNP polymer matrix.
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Acknowledgments
The authors gratefully acknowledge the financial support of this study by Project European IRSES-318524-NANODEV. Furthermore, this study was partly supported by the NASU in the frame of Scientific and Technical Program “Sensor devices for medical-ecological and industrial purposes: metrology and trial performance.” I. Kucherenko thanks the French Ministry of Higher Education and Research and Campus France for his PhD grant.
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Kucherenko, I.S. et al. (2017). Gold Nanoparticle/Polymer/Enzyme Nanocomposite for the Development of Adenosine Triphosphate Biosensor. In: Fesenko, O., Yatsenko, L. (eds) Nanophysics, Nanomaterials, Interface Studies, and Applications . NANO 2016. Springer Proceedings in Physics, vol 195. Springer, Cham. https://doi.org/10.1007/978-3-319-56422-7_39
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DOI: https://doi.org/10.1007/978-3-319-56422-7_39
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