Abstract
Protein-based stable isotope probing (protein-SIP) was developed to link microbial-specific metabolic function to phylogenetic information. The principle of the SIP-concept is the supplement of stable isotope-labelled compounds resulting in the labelling of microorganisms that are capable of utilizing these substrates as carbon source. The sum of all proteins reflects the functional status of the living organisms, so that the degree of heavy isotope incorporation represents a proxy for substrate assimilation and their activity. The main focus of this chapter is on the application of protein-SIP to elucidate metabolic processes in general and in particular those involved in the anaerobic degradation of aromatic hydrocarbons. Thus, the application of protein-SIP is a useful method to investigate the composition and the functional state of microbial communities.
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Jehmlich, N., von Bergen, M. (2020). Protein-Based Stable Isotope Probing (Protein-SIP): Applications for Studying Aromatic Hydrocarbon Degradation in Microbial Communities. In: Boll, M. (eds) Anaerobic Utilization of Hydrocarbons, Oils, and Lipids. Handbook of Hydrocarbon and Lipid Microbiology . Springer, Cham. https://doi.org/10.1007/978-3-319-50391-2_17
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DOI: https://doi.org/10.1007/978-3-319-50391-2_17
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