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TILLING and EcoTILLING for Discovery of Induced and Natural Variations in Sorghum Genome

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The Sorghum Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

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Abstract

TILLING is a reverse-genetic method to uncover the induced variation from the mutagenized population. Initially induced variation was detected by melting and reannealing the PCR products, cleavage of heteroduplex using CEL I endonucleases, and the resulting products separated and visualized on sequencing gels or capillaries. Subsequent sequence analysis in heteroduplex regions of individual plant DNA identified the mutation. In the past 6 years, mutation discovery through sequencing is becoming popular. Sequencing-based TILLING is less labor intensive with high specificity and confidence of mutation discovery. TILLING by sequencing has been reported in many crop plants including rice and wheat and is yet to be applied in sorghum. A conventional TILLING project has been reported in sorghum for agronomically important traits and also for the development of acyanogenic or low cyanogenic mutants in sorghum. EcoTILLING is another reverse-genetic approach that utilizes the same principles in the natural population instead of the mutagenized population. In this chapter, we discuss TILLING and EcoTILLING in sorghum and also TILLING by sequencing for the improvement of sorghum through the reverse-genetic approach.

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Authors and Affiliations

  1. Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Ramadoss Bharathi Raja & Ravindra N. Chibbar

  2. University of Agricultural Sciences, Bangalore, 560065, Karnataka, India

    Somanath Agasimani

  3. Department of Plant Genetic Resources, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641003, TN, India

    Ramadoss Bharathi Raja, Varadharaju Anusheela, Venkatesan Thiruvengadam & Sundaram Ganesh Ram

Authors
  1. Ramadoss Bharathi Raja
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  2. Somanath Agasimani
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  3. Varadharaju Anusheela
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  4. Venkatesan Thiruvengadam
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  5. Ravindra N. Chibbar
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  6. Sundaram Ganesh Ram
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Correspondence to Sundaram Ganesh Ram .

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Editors and Affiliations

  1. Indian Institute of Millets Research , Hyderabad, Andhra Pradesh, India

    Sujay Rakshit

  2. Department of Biology, University of Louisiana at Lafayette, Lafayette, USA

    Yi-Hong Wang

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Bharathi Raja, R., Agasimani, S., Anusheela, V., Thiruvengadam, V., Chibbar, R.N., Ganesh Ram, S. (2016). TILLING and EcoTILLING for Discovery of Induced and Natural Variations in Sorghum Genome. In: Rakshit, S., Wang, YH. (eds) The Sorghum Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-319-47789-3_14

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