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Somatic Embryogenesis in Temporary Immersion Bioreactors

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Book cover Somatic Embryogenesis: Fundamental Aspects and Applications

Abstract

Somatic embryogenesis is a very useful micropropagation technique, due to its high multiplicative capacity that offers the potential for large-scale propagation using temporary immersion bioreactors. The temporary immersion system is mainly based on the contact of plant tissue with culture medium by certain cycles of immersion, avoiding the problems of hyperhydricity, malformed embryos, and low conversion rates, which occur in continuous immersion systems. The automation of some or all of the phases of the process of somatic embryogenesis in a bioreactor could reduce labor and gellant costs and increase micropropagation efficiency, allowing high-quality plantlets to be obtained through more efficient and controlled protocols. This chapter describes the different types of temporary immersion bioreactors that have been used to increase or scale somatic embryogenesis in different plant species.

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Authors and Affiliations

  1. Unidad Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 no. 130, Col. Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, Mexico

    Kelly Maribel Monja-Mio, Miguel Ángel Herrera-Alamillo & Manuel L. Robert

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  1. Kelly Maribel Monja-Mio
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  2. Miguel Ángel Herrera-Alamillo
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  3. Manuel L. Robert
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Correspondence to Manuel L. Robert .

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Editors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Víctor M Loyola-Vargas

  2. Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Mexico

    Neftalí Ochoa-Alejo

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Monja-Mio, K.M., Herrera-Alamillo, M.Á., Robert, M.L. (2016). Somatic Embryogenesis in Temporary Immersion Bioreactors. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Somatic Embryogenesis: Fundamental Aspects and Applications. Springer, Cham. https://doi.org/10.1007/978-3-319-33705-0_24

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