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Chymotrypsin Probe for Real-Time Visualization of Pancreatic Leak

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Book cover Fluorescence Imaging for Surgeons

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Abstract

Pancreatic fistula remains the most serious complication after digestive surgery. This is mainly because there are no techniques to identify pancreatic leak from the pancreatic stump during surgery. Nor have there been any techniques for rapid evaluation of protease activity in leaking fluid during surgery that can cause severe tissue damage. We have designed and developed a novel fluorescence probe (glutaryl phenylalanine hydroxymethyl rhodamine green) that is activated by chymotrypsin. The use of this novel probe is expected to result in a reduced incidence of pancreatic fistula and operative mortality after digestive surgery.

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Financial Disclosure: The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in this manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, and royalties. No writing assistance was used in the production of this manuscript.

Funding/Support: This work was supported by grants from the Takeda Science Foundation, the Ministry of Education, Culture, Sports, Science, Technology of Japan, and the Ministry of Health, Labour, and Welfare of Japan, and Global Leader Program for Social Design and Management.

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Authors and Affiliations

  1. Department of Surgery, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Suguru Yamashita M.D.

  2. Department of Gastroenterological Surgery, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Koto-ku, Tokyo, Japan

    Takeaki Ishizawa M.D., Ph.D., F.A.C.S.

  3. Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Takeaki Ishizawa M.D., Ph.D., F.A.C.S. & Norihiro Kokudo M.D., Ph.D.

  4. Laboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Masayo Sakabe Ph.D., Mako Kamiya Ph.D. & Yasuteru Urano Ph.D.

Authors
  1. Suguru Yamashita M.D.
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  2. Takeaki Ishizawa M.D., Ph.D., F.A.C.S.
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  3. Masayo Sakabe Ph.D.
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  4. Mako Kamiya Ph.D.
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  5. Yasuteru Urano Ph.D.
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  6. Norihiro Kokudo M.D., Ph.D.
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Corresponding authors

Correspondence to Yasuteru Urano Ph.D. or Norihiro Kokudo M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Section of Minimally Invasive Surgery, Department of General and Vascular Surgery, The Bariatric and Metabolic Institute, Cleveland Clinic Florida, FL, USA; Oncological Surgical Division, Division of Surgical Research, Department of Surgery, Hospital de Clinicas Buenos Aires, University of Buenos Aires, Buenos Aires, Argentina

    Fernando D. Dip

  2. Department of Gastroenterological Surgery, Cancer Institute Hospital, Japanese Foundation for Cancer Research; Hepato-Biliary-Pancreatic Surgery, Department of Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan

    Takeaki Ishizawa

  3. Hepato-Biliary-Pancreatic Surgery; Department of Surgery, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Norihiro Kokudo

  4. Department of General Surgery, Section of Minimally Invasive Surgery; Department of General and Vascular Surgery, The Bariatric and Metabolic Institute, Cleveland Clinic Florida, Weston, Florida, USA

    Raul J. Rosenthal

Electronic Supplementary Material

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Fluorescence imaging of leaking pancreatic juice using the chymotrypsin probe. Fluorescence imaging clearly visualized leakage of pancreatic juice along a stapler line beginning 3 min after spraying chymotrypsin probe on the pancreatic stump (WMV 39,304 kb)

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© 2015 Springer International Publishing Switzerland

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Yamashita, S., Ishizawa, T., Sakabe, M., Kamiya, M., Urano, Y., Kokudo, N. (2015). Chymotrypsin Probe for Real-Time Visualization of Pancreatic Leak. In: Dip, F., Ishizawa, T., Kokudo, N., Rosenthal, R. (eds) Fluorescence Imaging for Surgeons. Springer, Cham. https://doi.org/10.1007/978-3-319-15678-1_36

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  • DOI: https://doi.org/10.1007/978-3-319-15678-1_36

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-15677-4

  • Online ISBN: 978-3-319-15678-1

  • eBook Packages: MedicineMedicine (R0)

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