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Protoplasts and Spheroplasts of Gram-Negative Bacteria (With Special Emphasis on Proteus mirabilis)

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Protoplasts 1983

Part of the book series: EXS 46: Experientia Supplementum ((EXS,volume 46))

Abstract

Cell wall-defective spherical forms of Gram-negative bacteria have a long tradition as tools in studies on bacterial biochemistry and molecular biology, and especially as host organisms for genetic transformation and transfection. Important organisms which have been used for such purposes include Escherichia coli, and several other enterobacteriaceae (Salmonella typhimurium, Shigella sp., Enterobacter aerogenes, Klebsiella pneumoniae, Serratia marcescens, Proteus sp.) in addition to Pseudomonas aeruginosa and Rhizobium meliloti (for review see McQuillen 1960, Martin 1963, Benzinger 1978). As a timely extension of the transfection and transformation studies, Tsenin et al. (1978) and Coetzee and his colleagues (1979) have delivered first reports on successful use of cell wall defective spheroplasts of Gram-negative bacteria E. coli and Providencia alcalifaciens for cell fusion followed by genetic recombination.

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Authors and Affiliations

  1. Institut für Mikrobiologie, Fachbereich 10 -Biologie-, Technische Hochschule Darmstadt, Schnittspahnstrasse 9, D-6100, Darmstadt, Germany

    H. H. Martin

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  1. H. H. Martin
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Editors and Affiliations

  1. Basel, Switzerland

    I. Potrykus , A. Hinnen , P. J. King  & R. D. Shillito , ,  & 

  2. Zürich, Switzerland

    C. T. Harms  & R. Hütter  & 

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Martin, H.H. (1983). Protoplasts and Spheroplasts of Gram-Negative Bacteria (With Special Emphasis on Proteus mirabilis). In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 46: Experientia Supplementum, vol 46. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6776-4_26

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  • DOI: https://doi.org/10.1007/978-3-0348-6776-4_26

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-6777-1

  • Online ISBN: 978-3-0348-6776-4

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