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Plant Cell and Tissue Differentiation and Secondary Metabolites pp 1–29Cite as

Propagation of Southern Sweet-Grass Using In Vitro Techniques as a Method for the Production of Plants Being a Source of Standardized Raw Material

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Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

Southern sweet-grass, popularly called bison grass, is one of the few aromatic grasses used for industrial purposes. The plant, found only in the northeastern part of Europe, is considered as threatened with extinction, which in certain countries has already led to its legal protection. However, wild-growing plants are still harvested and used as a source of raw material, i.e., leaves applied for aromatization of alcoholic beverages, as well as in tobacco and food products. Therefore, it seems that attempts should be made to bring this species into cultivation. Such practices, focused mainly on the production of the required amount of leaves for industry, also promote the regeneration of plant populations on natural sites, made possible due to the lower price of raw material collected from cultivation. One of the biggest problems encountered when introducing wild-growing plants into cultivation is their propagation and especially low seed germination or viability. Propagation of southern sweet-grass using seeds is inefficient; their germination rate is very low and the process uneven. Moreover, this method provides highly diversified reproductive plant material, which in turn is not attractive for cultivation since the raw material obtained from such plants does not meet the expectations of industry regarding quality standards. Micropropagation of southern sweet-grass by indirect regeneration with the use of immature inflorescences as initial explants seems to be a promising source of reproductive material for establishing plantations. This method allows the cultivation of selected clones or forms of the desired chemical profile and in consequence provides raw material which exceeds the quality of raw material derived from wild-growing plants.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

ABA:

Abscisic acid

BA:

6-Benzyladenine

DW:

Dry weight

GA3:

Gibberellic acid

HPLC :

High-performance liquid chromatography

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KNO3:

Potassium nitrate

MAPs:

Medicinal and aromatic plants

MS:

Murashige and Skoog medium

NAA:

1-Naphthaleneacetic acid

TDZ:

Thidiazuron

WR:

Without growth regulators

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Acknowledgments

The work was supported by National Science Centre, project No. N N310 728440.

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Authors and Affiliations

  1. Laboratory of New Herbal Products, Department of Vegetable and Medicinal Plants, Warsaw University of Life Sciences – SGGW, Warsaw, Poland

    Katarzyna Bączek, Anna Pawełczak, Ewelina Pióro-Jabrucka, Jarosław L. Przybył, Olga Kosakowska & Zenon Węglarz

Authors
  1. Katarzyna Bączek
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  2. Anna Pawełczak
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  3. Ewelina Pióro-Jabrucka
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  4. Jarosław L. Przybył
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  5. Olga Kosakowska
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  6. Zenon Węglarz
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Corresponding author

Correspondence to Zenon Węglarz .

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Editors and Affiliations

  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Bączek, K., Pawełczak, A., Pióro-Jabrucka, E., Przybył, J.L., Kosakowska, O., Węglarz, Z. (2019). Propagation of Southern Sweet-Grass Using In Vitro Techniques as a Method for the Production of Plants Being a Source of Standardized Raw Material. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_28-1

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  • DOI: https://doi.org/10.1007/978-3-030-11253-0_28-1

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  • Print ISBN: 978-3-030-11253-0

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