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Monoclonal Antibodies pp 133–149Cite as

Selection and Screening Using Antibody Phage Display Libraries

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1131))

Abstract

Phage display is a powerful tool to isolate specific binders from a large and diverse combinatorial library. Here we provide a step-by-step protocol in how to set up a successful phage panning experiment in order to isolate useful antibodies. The protocol includes testing antigens for their suitability in the phage panning procedure and optimizing the panning conditions and alternative screening methods to minimize nonspecific binding. We describe example phage panning experiments starting from the library transformation to the phage clone screening.

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Acknowledgment

We thank Alessandro Palumbo, Sarah Sanowar, Jack Lin, and C. Vivian Lee for contributing to the development of protocols.

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Authors and Affiliations

  1. Antibody Engineering Department, Genentech Inc., South San Francisco, CA, USA

    Patrick Koenig & Germaine Fuh

Authors
  1. Patrick Koenig
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  2. Germaine Fuh
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Editor information

Editors and Affiliations

  1. Département de Biochimie, University of Geneva, Geneva, Switzerland

    Vincent Ossipow

  2. NovImmune SA, Plan-les-Ouates, Switzerland

    Nicolas Fischer

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Koenig, P., Fuh, G. (2014). Selection and Screening Using Antibody Phage Display Libraries. In: Ossipow, V., Fischer, N. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 1131. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-992-5_9

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  • DOI: https://doi.org/10.1007/978-1-62703-992-5_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-991-8

  • Online ISBN: 978-1-62703-992-5

  • eBook Packages: Springer Protocols

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