Abstract
The opsonophagocytic killing (OPK) assay is used as a correlate for protection to measure the functional capacities of vaccine-candidate-raised antibodies. This in vitro assay aids selecting promising vaccines by demonstrating whether the vaccine-induced antibodies drive efficient complement deposition and subsequent opsonophagocytic killing. Here, we describe two protocols for an OPK assay using either human-derived PMNs or cultured HL-60 cells.
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References
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Dwyer, M., Gadjeva, M. (2014). Opsonophagocytic Assay. In: Gadjeva, M. (eds) The Complement System. Methods in Molecular Biology, vol 1100. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-724-2_32
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DOI: https://doi.org/10.1007/978-1-62703-724-2_32
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-723-5
Online ISBN: 978-1-62703-724-2
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