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Detection of Soluble CR3 (CD11b/CD18) by Time-Resolved Immunofluorometry

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The Complement System

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1100))

Abstract

In the cell membrane complement receptor 3 (CR3) consists of one alpha chain (CD11b) and one beta chain (CD18). CR3 participates in many immunological processes, especially those involving cell migration, adhesion, and phagocytosis of complement-opsonized microbes. Recent findings of soluble CR3 in body fluids and in culture supernatant from experiments in vitro point to the involvement of ecto domain shedding as a part of the CR3 biology. To monitor such shedding on a quantitative basis, we have developed time-resolved immunofluorometric assays (TRIFMA) to detect soluble CD11b and CD18 in plasma or serum of either human or murine origin. Compared with most enzyme-linked immunosorbent assays methodologies, TRIFMA possesses prominent advantages, including better dynamic range and reproducibility. These assays may contribute to the understanding of the role of shedding of CR3 and other cell adhesion molecules in human disease and animal models involving inflammation.

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Acknowledgements

We would like to thank Drs. Jens C. Jensenius and Steffen Thiel, Dept. of Biomedicine, Aarhus University, for their support and excellent advice on applications of TRIFMA. This work was financially supported by grants from the Lundbeck Foundation (LUNA), the Danish Multiple Sclerosis Association, and the Danish Council for Independent Research | Medical Sciences.

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Authors and Affiliations

  1. Biophysical Immunology Laboratory, Department of Biomedicine, The Lundbeck Foundation Nanomedicine Center for Individual Management of Tissue Damage and Regeneration, Aarhus University, Aarhus, Denmark

    Gitte Krogh Nielsen

  2. Biophysical Immunology Laboratory, Department of Biomedicine, The Lundbeck Foundation Nanomedicine Center for Individualized Management of Tissue Damage and Regeneration, and Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark

    Thomas Vorup-Jensen

Authors
  1. Gitte Krogh Nielsen
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  2. Thomas Vorup-Jensen
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Editors and Affiliations

  1. Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard University Harvard Medical School, Boston, Massachusetts, USA

    Mihaela Gadjeva

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Nielsen, G.K., Vorup-Jensen, T. (2014). Detection of Soluble CR3 (CD11b/CD18) by Time-Resolved Immunofluorometry. In: Gadjeva, M. (eds) The Complement System. Methods in Molecular Biology, vol 1100. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-724-2_30

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  • DOI: https://doi.org/10.1007/978-1-62703-724-2_30

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-723-5

  • Online ISBN: 978-1-62703-724-2

  • eBook Packages: Springer Protocols

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