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Bioluminescent Imaging pp 249–258Cite as

Noninvasive In Vivo Monitoring of Extracellular Vesicles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1098))

Abstract

Extracellular vesicles (EVs) including exosomes and microvesicles are nanometer-sized vesicles released by cells to deliver lipids, cellular proteins, mRNAs, and noncoding RNAs, thereby facilitating intercellular communication without direct cell-to-cell contacts. Due to their nanoscale size, EVs have been visualized under microscopy in vitro. We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo.

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Acknowledgement

We wish to thank Mr. Osama Mardini for his technical assistance. This work was supported by NIH grant NIH/NCI CA069246, CA141150 and Canadian Institutes of Health Research (CIHR).

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Authors and Affiliations

  1. Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA

    Charles P. Lai & Xandra O. Breakefield

  2. Program in Neuroscience, Harvard Medical School, Boston, MA, USA

    Charles P. Lai, Bakhos A. Tannous & Xandra O. Breakefield

  3. Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA

    Bakhos A. Tannous

  4. Department of Radiology, Center for Molecular Imaging Research, Massachusetts General Hospital, Boston, MA, USA

    Xandra O. Breakefield

Authors
  1. Charles P. Lai
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  2. Bakhos A. Tannous
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  3. Xandra O. Breakefield
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Editor information

Editors and Affiliations

  1. Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, Massachusetts, USA

    Christian E. Badr

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Lai, C.P., Tannous, B.A., Breakefield, X.O. (2014). Noninvasive In Vivo Monitoring of Extracellular Vesicles. In: Badr, C. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 1098. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-718-1_19

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  • DOI: https://doi.org/10.1007/978-1-62703-718-1_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-717-4

  • Online ISBN: 978-1-62703-718-1

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