Abstract
In vivo bioluminescence imaging (BLI) is a powerful technology that gives information on biological processes in living animals over multiple time points. Importantly BLI can also yield anatomical localization of signal which can provide important information when performing biodistribution studies of different macromolecules. This is of particular interest for gene therapy vectors such as adeno-associated virus (AAV) vectors in which knowledge of in vivo gene expression profiles help characterize what target tissues or organs the vector may be useful for. It can also be utilized to assess novel vector systems for their ability to overcome specific in vivo barriers of effective gene therapy. Here we describe BLI of AAV-encoded firefly luciferase (Fluc) expression in mice after intravascular delivery. This protocol can be amended for use with different virus vectors (e.g., lentivirus, adenovirus) as well as nonviral gene delivery (e.g., plasmid DNA, liposomes).
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Acknowledgement
This work was supported by NIH/NINDS 1 R21 NS081374-01. CM has a financial interest in Exosome Diagnostics, Inc. CM’s interests were reviewed and are managed by the Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies.
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Maguire, C.A. (2014). Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice. In: Badr, C. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 1098. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-718-1_16
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DOI: https://doi.org/10.1007/978-1-62703-718-1_16
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-718-1
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