Abstract
The imaging of spontaneously occurring tumors in mice poses many technical and logistical problems. Recently a mouse model was generated in which a chimeric protein consisting of HIF-1α oxygen-dependent degradation domain (ODD) fused to luciferase was ubiquitously expressed in all tissues. Hypoxic stress leads to the accumulation of ODD-luciferase in the tissues of this mouse model which can be identified by noninvasive bioluminescence measurement. Crossing this transgenic mouse with tumorigenic mice yields solid tumors with hypoxic cores that may be successfully imaged and characterized using the technique described herein.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Burns S (2004) Oncology: tumors and treatment—a photographic history, 1845–1945. Burns Archive Press, New York, NY
Dalla Palma L, Pozzi Mucelli RS, Ricci C, Zuiani C (1989) Ultrasonography in oncology. A review. Acta Oncol 28:157–162
Bragg DG (1977) Advances in diagnostic radiology: problems and prospects. Cancer 40:500–508
Hawkins RA, Phelps ME (1988) PET in clinical oncology. Cancer Metastasis Rev 7:119–142
Frank JA, Dwyer AJ, Doppman JL (1987) Nuclear magnetic resonance imaging in oncology. Important. Adv Oncol: 133–174
Mansfield CM, Park CH (1985) Contribution of radionuclide imaging to radiation oncology. Semin Nucl Med 15:28–45
Dennis C (2006) Cancer: off by a whisker. Nature 442:739–741
Sharpless NE, Depinho RA (2006) The mighty mouse: genetically engineered mouse models in cancer drug development. Nat Rev Drug Discov 5:741–754
Ince TA, Richardson AL, Bell GW et al (2007) Transformation of different human breast epithelial cell types leads to distinct tumor phenotypes. Cancer Cell 12:160–170
Shen Q, Brown PH (2005) Transgenic mouse models for the prevention of breast cancer. Mutat Res 576:93–110
Muller WJ (1991) Expression of activated oncogenes in the murine mammary gland: transgenic models for human breast cancer. Cancer Metastasis Rev 10:217–227
Leenders MW, Nijkamp MW, Borel Rinkes IH (2008) Mouse models in liver cancer research: a review of current literature. World J Gastroenterol 14:6915–6923
Jeet V, Russell PJ, Khatri A (2010) Modeling prostate cancer: a perspective on transgenic mouse models. Cancer Metastasis Rev 29:123–142
Guy CT, Webster MA, Schaller M et al (1992) Expression of the neu protooncogene in the mammary epithelium of transgenic mice induces metastatic disease. Proc Natl Acad Sci U S A 89:10578–10582
Muller WJ, Arteaga CL, Muthuswamy SK et al (1996) Synergistic interaction of the Neu proto-oncogene product and transforming growth factor alpha in the mammary epithelium of transgenic mice. Mol Cell Biol 16:5726–5736
Hwangdo W, Ko HY, Lee JH et al (2010) A nucleolin-targeted multimodal nanoparticle imaging probe for tracking cancer cells using an aptamer. J Nucl Med 51:98–105
Tennant DA, Duran RV, Gottlieb E (2010) Targeting metabolic transformation for cancer therapy. Nat Rev Cancer 10:267–277
Koh MY, Spivak-Kroizman TR, Powis G (2010) HIF-1alpha and cancer therapy. Recent Results Cancer Res 180:15–34
Michieli P (2009) Hypoxia, angiogenesis and cancer therapy: to breathe or not to breathe? Cell Cycle 8:3291–3296
Harris AL (2002) Hypoxia–a key regulatory factor in tumour growth. Nat Rev Cancer 2:38–47
Safran M, Kim WY, O’Connell F et al (2006) Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: assessment of an oral agent that stimulates erythropoietin production. Proc Natl Acad Sci U S A 103:105–110
Goldman SJ, Chen E, Taylor R, Petrosky W et al (2011) Use of the ODD-luciferase transgene for the non-invasive imaging of spontaneous tumors in mice. PLoS One 6(3):e18269
Keyaerts M, Remory I, Caveliers V et al (2012) Inhibition of firefly luciferase by general anesthetics: effect on in vitro and in vivo bioluminescence imaging. PLoS One 7(1):e30061
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Goldman, S.J., Jin, S. (2014). The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice. In: Badr, C. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 1098. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-718-1_11
Download citation
DOI: https://doi.org/10.1007/978-1-62703-718-1_11
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-717-4
Online ISBN: 978-1-62703-718-1
eBook Packages: Springer Protocols