Abstract
Different plant cell types express unique transcriptomes, proteomes, and metabolomes. Therefore, the isolation of specific cell types prior to molecular analyses is important to understand the specification, differentiation, and function of these cells. Isolation of specific plant cell types from composite organs can be achieved by laser microdissection (LMD). A wide variety of methods to fix and embed tissues prior to LMD and downstream molecular analyses have been developed for different plant species and tissues. The present review summarizes and highlights the most recently applied LMD approaches in plant science.
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References
Schnable PS, Hochholdinger F, Nakazono M (2004) Global expression profiling applied to plant development. Curr Opin Plant Biol 7:50–56
Woll K, Borsuk LA, Stransky H et al (2005) Isolation, characterization, and pericycle-specific transcriptome analyses of the novel maize lateral and seminal root initiation mutant rum1. Plant Physiol 139:1255–1267
Dembinsky D, Woll K, Saleem M et al (2007) Transcriptomic and proteomic analyses of pericycle cells of the maize primary root. Plant Physiol 145:575–588
Nestler J, Schütz W, Hochholdinger F (2011) Conserved and unique features of the maize (Zea mays L.) root hair proteome. J Proteome Res 10:2525–2537
McDowell ET, Kapteyn J, Schmidt A et al (2011) Comparative functional genomic analysis of Solanum glandular trichome types. Plant Physiol 155:524–539
Birnbaum K, Shasha DE, Wang JY et al (2003) A gene expression map of the Arabidopsis root. Science 302:1956–1960
Kerk NM, Ceserani T, Tausta SL (2003) Laser capture microdissection of cells from plant tissues. Plant Physiol 132:27–35
Nakazono M, Qiu F, Borsuk LA et al (2003) Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell 15:583–596
Barcala M, Fenoll C, Escobar C (2012) Laser microdissection of cells and isolation of high-quality RNA after cryosectioning. Methods Mol Biol 883:87–95
Cai S, Lashbrook CC (2006) Laser capture microdissection of plant cells from tape-transferred paraffin sections promotes recovery of structurally intact RNA for global gene profiling. Plant J 48:628–637
Liu Y, von Behrens I, Muthreich N et al (2010) Regulation of the pericycle proteome in maize (Zea mays L.) primary roots by RUM1 which is required for lateral root initiation. Eur J Cell Biol 89:236–241
Kaspar S, Weier D, Weschke W et al (2010) Protein analysis of laser capture micro-dissected tissues revealed cell-type-specific biological functions in developing barley grains. Anal Bioanal Chem 398:2883–2893
Takehisa H, Sato Y, Igarashi M et al (2012) Genome-wide transcriptome dissection of the rice root system: implications for developmental and physiological functions. Plant J 69: 126–140
Brooks L III, Strable J, Zhang X et al (2009) Microdissection of shoot meristem functional domains. PLoS Genet 5:e1000476
Suwabe K, Suzuki G, Takahashi H et al (2008) Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray. Plant Cell Physiol 49:1407–1416
Takahashi H, Kamakura H, Sato Y et al (2010) A method for obtaining high quality RNA from paraffin sections of plant tissues by laser microdissection. J Plant Res 123:807–813
Ishimaru T, Nakazono M, Masumura T et al (2007) A method for obtaining high integrity RNA from developing aleurone cells and starchy endosperm in rice (Oryza sativa L.) by laser microdissection. Plant Sci 173:321–326
Inada N, Wildermuth MC (2005) Novel tissue preparation method and cell-specific marker for laser microdissection of Arabidopsis mature leaf. Planta 221:9–16
Zhang X, Madi S, Borsuk L et al (2007) Laser microdissection of narrow sheath mutant maize uncovers novel gene expression in the shoot apical meristem. PLoS Genet 3:e101
Emrich SJ, Barbazuk WB, Li L et al (2007) Gene discovery and annotation using LCM-454 transcriptome sequencing. Genome Res 17:69–73
Schichnes D, Nemson J, Sohlberg L et al (1998) Microwave protocols for paraffin microtechnique and in situ localization in plants. Microsc Microanal 4:491–496
Kobayashi K, Yasuno N, Sato Y et al (2012) Inflorescence meristem identity in rice is specified by overlapping functions of three AP1/FUL-like MADS box genes and PAP2, a SEPALLATA MADS box gene. Plant Cell 24:1848–1859
Schiebold S, Tschiersch H, Borisjuk L et al (2011) A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus. Plant Methods 7:19
Agusti J, Merelo P, Cercos M et al (2009) Comparative transcriptional survey between laser-microdissected cells from laminar abscission zone and petiolar cortical tissue during ethylene-promoted abscission in citrus leaves. BMC Plant Biol 9:127
Spencer MWB, Casson SA, Lindsey K (2007) Transcriptional profiling of the Arabidopsis embryo. Plant Physiol 143:924–940
Eberwine J, Yeh H, Miyashiro K et al (1992) Analysis of gene expression in single live neurons. Proc Natl Acad Sci USA 89: 3010–3014
Gaude N, Bortfeld S, Duensing N et al (2012) Arbuscule-containing and non-colonized cortical cells of mycorrhizal roots undergo extensive and specific reprogramming during arbuscular mycorrhizal development. Plant J 69: 510–528
Luo L, Salunga RC, Guo H et al (1999) Gene expression profiles of laser-captured adjacent neuronal subtypes. Nat Med 5: 117–122
Acknowledgment
Root research in the laboratory of FH is supported by the DFG (Deutsche Forschungsgemeinschaft) and the 7th Framework Programme of the European Union.
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Ludwig, Y., Hochholdinger, F. (2014). Laser Microdissection of Plant Cells. In: Žárský, V., Cvrčková, F. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1080. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-643-6_21
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DOI: https://doi.org/10.1007/978-1-62703-643-6_21
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