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Gel Electrophoresis-Based Proteomics of Senescent Tissues

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Biological Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1048))

Abstract

Cellular aging is a fundamental biological process, and mass spectrometry-based proteomics has been widely used for the global identification of age-related changes in a variety of tissues. The proteomic profiling of senescent skeletal muscles has revealed a variety of alterations in proteins associated with the contractile apparatus, cell signaling, ion homeostasis, metabolism, and the cellular stress response. Here, we outline the two-dimensional gel electrophoretic separation and fluorescent labeling of the urea-soluble protein complement from aged diaphragm muscle. This chapter describes the various experimental steps involved in gel electrophoresis-based proteomics, including protein extraction, isoelectric focusing, slab gel electrophoresis, fluorescence labeling, image analysis, protein digestion, mass spectrometric identification of proteins and immunoblotting.

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Authors and Affiliations

  1. Department of Biology, National University of Ireland Maynooth, Maynooth, Kildare, Ireland

    Steven Carberry & Kay Ohlendieck

Authors
  1. Steven Carberry
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  2. Kay Ohlendieck
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Editors and Affiliations

  1. Dept. Biology, University of Alabama, Birmingham Dept. Biology, Birmingham, Alabama, USA

    Trygve O. Tollefsbol

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Carberry, S., Ohlendieck, K. (2013). Gel Electrophoresis-Based Proteomics of Senescent Tissues. In: Tollefsbol, T. (eds) Biological Aging. Methods in Molecular Biology, vol 1048. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-556-9_17

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  • DOI: https://doi.org/10.1007/978-1-62703-556-9_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-555-2

  • Online ISBN: 978-1-62703-556-9

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