Abstract
Transgenic expression of catalase in mitochondria using a transgenic strategy extends life span and prevents aging-related pathology in mice. However, transgenic overexpression is not suitable for a clinical application. Adeno-associated virus (AAV) is the most promising gene delivery vehicle. Here we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT on muscle contractility and running performance in mice.
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Acknowledgments
The studies are supported by grants from the National Institutes of Health AR-49419 and the Muscular Dystrophy Association. We thank Dr. James Wilson (University of Pennsylvania, Philadelphia, PA) for providing the AAV-9 packaging plasmid.
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Li, D., Duan, D. (2013). Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors. In: Tollefsbol, T. (eds) Biological Aging. Methods in Molecular Biology, vol 1048. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-556-9_13
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DOI: https://doi.org/10.1007/978-1-62703-556-9_13
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