Abstract
Invadopodia are specialized structures of cancer cells which aid in cancer cell invasion and metastasis. Therefore, studying the early steps of invadopodium assembly and its life cycle at the subcellular level by using high spatiotemporal resolution imaging provides an opportunity for understanding the signaling mechanisms involved in this very important process. In this chapter, we describe the design of a custom-built high-resolution fluorescence microscope which makes this challenging imaging possible. We also describe an ImageJ plugin that we have developed for tracking of invadopodia and lifetime analysis.
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Acknowledgments
We thank Dr. Louis Hodgson, and members of the Analytical Imaging Facility and Gruss-Lipper Biophotonics Center for helping in the microscope design. We also thank people from Condeelis, Segall, and Cox laboratories for helpful discussions. This work was supported by a postdoctoral fellowship to Ved Sharma from Susan G. Komen for the Cure© (KG111405), the Integrated Imaging Program and CA150344.
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Sharma, V.P., Entenberg, D., Condeelis, J. (2013). High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis. In: Coutts, A. (eds) Adhesion Protein Protocols. Methods in Molecular Biology, vol 1046. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-538-5_21
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DOI: https://doi.org/10.1007/978-1-62703-538-5_21
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