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Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes

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Mouse Models of Allergic Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1032))

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Abstract

Transcript profiling reveals valuable insights to molecular and cellular activity related to disease. Gene expression profiles provide clues as to how tissues or cells in a particular environment may respond to stimuli. Gene-targeted examination of transcript changes is accomplished by employing a quantitative PCR approach using cDNA prepared from isolated RNA.

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Acknowledgments

This work was supported by National Institute of Allergy and Infectious Disease grant U19-AI077437 and Radiation Countermeasures Center of Research Excellence (RadCCORE) subcontract U19-AI067798. The technical expertise of Michael Thompson and Karen McKinnon is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Willie June Brickey

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  1. Willie June Brickey
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Editor information

Editors and Affiliations

  1. Dept. of Biomedical Sciences & Pathobiol, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    Irving C. Allen

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Brickey, W.J. (2013). Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_22

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  • DOI: https://doi.org/10.1007/978-1-62703-496-8_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-495-1

  • Online ISBN: 978-1-62703-496-8

  • eBook Packages: Springer Protocols

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