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Mouse Models of Allergic Disease pp 271–286Cite as

Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1032))

Abstract

The increased incidence of asthma over the last 50 years in developed countries has been associated with a decrease in infections acquired early in childhood. These early infections are thought to shape subsequent immune responses. Although there have been multiple clinical associations between gastrointestinal infections and decreased asthma incidence, it has been difficult to move beyond a simple correlation when studying human patients. This section describes an acute asthma model in C57BL/6 mice designed to specifically evaluate the effect of prior gastric Helicobacter colonization and inflammation in a murine model of cockroach allergen-induced asthma.

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Acknowledgments

The authors would like to thank Kim Estell for assistance with airway hyperresponsiveness analysis and Ben Christmann for helping with the lung inflammation technique. We would also like to thank J. McNaught for slide preparation and M. Harris for animal husbandry, and members of the Lorenz lab for valuable advice. This study was supported in part by NIH grants R01 DK059911; P01 DK071176; the American Asthma Foundation grant 06-0167; and University of Alabama at Birmingham Digestive Diseases Research Development Center grant P30 DK064400. CGD is supported by the Howard Hughes Medical Institute Med into Grad Fellowship. Aspects of this project were conducted in biomedical research space that was constructed with funds supported in part by NIH grant C06RR020136.

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Authors and Affiliations

  1. Department of Medicine, University of North Carolina, Chapel Hill, NC, USA

    Carolyn G. Durham

  2. Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA

    Lisa M. Schwiebert

  3. Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA

    Robin G. Lorenz

Authors
  1. Carolyn G. Durham
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  2. Lisa M. Schwiebert
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  3. Robin G. Lorenz
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Editors and Affiliations

  1. Dept. of Biomedical Sciences & Pathobiol, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    Irving C. Allen

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Durham, C.G., Schwiebert, L.M., Lorenz, R.G. (2013). Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_21

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  • DOI: https://doi.org/10.1007/978-1-62703-496-8_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-495-1

  • Online ISBN: 978-1-62703-496-8

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