Abstract
Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. Here, we describe a series of procedures designed to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.
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Acknowledgements
This research was supported by the Intramural Research Program of the NIH, National Institute of Dental and Craniofacial Research.
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Masedunskas, A., Sramkova, M., Parente, L., Weigert, R. (2012). Intravital Microscopy to Image Membrane Trafficking in Live Rats. In: Taatjes, D., Roth, J. (eds) Cell Imaging Techniques. Methods in Molecular Biology, vol 931. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-056-4_9
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DOI: https://doi.org/10.1007/978-1-62703-056-4_9
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