Abstract
This protocol describes the generation of monoclonal antibodies in single-chain variable fragment (scFv)-Fc format. It includes the cloning of the scFv-Fc expression cassette into a mammalian expression vector followed by transient transfection of mammalian cells and purification by protein A affinity chromatography. The protocol is intended for applications in basic and preclinical research that require rapid access to milligram amounts of protein.
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Acknowledgments
This work was supported by the Intramural Research Program of the Center for Cancer Research, National Cancer Institute, National Institutes of Health. We thank Marko Modric for comments.
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Yang, J., Rader, C. (2012). Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format. In: Proetzel, G., Ebersbach, H. (eds) Antibody Methods and Protocols. Methods in Molecular Biology, vol 901. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-931-0_14
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DOI: https://doi.org/10.1007/978-1-61779-931-0_14
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-930-3
Online ISBN: 978-1-61779-931-0
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