Abstract
Second-generation DNA sequencing platforms have emerged as powerful tools in biological research. Their high sequence output at lower cost and minimal input DNA requirement render them suitable for broad applications ranging from gene expression studies to personalized clinical diagnostics. Here, we describe the preparation of cDNA libraries, from both whole aphid insects and their microscopic salivary gland tissues, suitable for high-throughput DNA sequencing on the Illumina platform.
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References
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Acknowledgment
This work is supported by USDA-NIFA grant 2009-05242 to IK.
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Atamian, H.S., Kaloshian, I. (2012). Construction of RNA-Seq Libraries from Large and Microscopic Tissues for the Illumina Sequencing Platform. In: Jin, H., Gassmann, W. (eds) RNA Abundance Analysis. Methods in Molecular Biology, vol 883. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-839-9_3
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DOI: https://doi.org/10.1007/978-1-61779-839-9_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-838-2
Online ISBN: 978-1-61779-839-9
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