Abstract
Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.
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References
Lysy PA, Smets F, Sibille C, Najimi M, Sokal EM (2007) Human skin fibroblasts: from mesodermal to hepatocyte-like differentiation. Hepatology 46:1574–1585
De Kock J, Vanhaecke T, Biernaskie J, Rogiers V, Snykers S (2009) Characterization and hepatic differentiation of skin-derived precursors from adult foreskin by sequential exposure to hepatogenic cytokines and growth factors reflecting liver development. Toxicol In Vitro 23:1522–1527
Toma JG, Akhavan M, Fernandes KJ, Barnabé-Heider F, Sadikot A, Kaplan DR, Miller FD (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784
Sieber-Blum M, Grim M, Hu YF, Szeder V (2004) Pluripotent neural crest stem cells in the adult hair follicle. Dev Dyn 231:258–269
Huang HI, Chen SK, Ling QD, Chien CC, Liu HT, Chan SH (2010) Multilineage differentiation potential of fibroblast-like stromal cells derived from human skin. Tissue Eng Part A 16:1491–1501
Aguiari P, Leo S, Zavan B, Vindigni V, Rimessi A, Bianchi K, Franzin C, Cortivo R, Rossato M, Vettor R, Abatangelo G, Pozzan T, Pinton P, Rizzuto R (2008) High glucose induces adipogenic differentiation of muscle-derived stem cells. Proc Natl Acad Sci U S A 105:1226–1231
Sekiya I, Larson BL, Vuoristo JT, Cui JG, Prockop DJ (2004) Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs). J Bone Miner Res 19:256–264
Gregory CA, Gunn WG, Peister A, Prockop DJ (2004) An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Anal Biochem 329:77–84
Zaminy A, Ragerdi Kashani I, Barbarestani M, Hedayatpour A, Mahmoudi R, Farzaneh NA (2008) Osteogenic differentiation of rat mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells: melatonin as a differentiation factor. Iran Biomed J 12:133–141
Penney DP, Powers JM, Frank M, Willis C, Churukian C (2002) Analysis and testing of biological stains—the Biological Stain Com-mission Procedures. Biotech Histochem 77: 237–275
Dulbecco R, Vogt M (1954) Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med 99(2):167–182
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Huang, HI., Wu, CZ. (2012). Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_27
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DOI: https://doi.org/10.1007/978-1-61779-815-3_27
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-814-6
Online ISBN: 978-1-61779-815-3
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