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Isolation, Culture, and Osteogenic/Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

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Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

Musculoskeletal disorders, as non-healing fractures and large bone defects, articular cartilage and subchondral bone injuries, often result in lifelong chronic pain and compromised quality of life. Although generally a natural process, failure of large bone defects to heal such as after complex fractures, resection of tumours, infections, or revisions of joint replacements remains a critical challenge that requires more appropriate solutions as those currently available. In addition, regeneration of chondral and osteochondral defects continues to be a challenge until to date. A profound understanding of the underlying mechanisms of endogenous regeneration is a prerequisite for successful bone and cartilage regeneration. Presently, one of the most promising therapeutic approaches is cell-based tissue engineering which provides a healthy population of cells to the injured site. Use of differentiated cells has severe limitations; an excellent alternative would be the application of adult marrow stromal cells/mesenchymal stem cells (MSC) which possess extensive proliferation potential and proven capability to differentiate along the osteochondral pathway. The process of osteo-/chondrogenesis can be mimicked in vitro by inducing osteo-chondroprogenitor stem cells to undergo osteogenesis and chondrogenesis through exposure of osteo-/chondrogenic favourable microenvironmental, mechanical, and nutritional conditions. This chapter provides comprehensive protocols for the isolation, expansion, and osteo-/chondrogenic differentiation of adult bone marrow-derived MSC.

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Authors and Affiliations

  1. Department of Orthopaedics, University of Regensburg and Centre for Biomedical Technology, BioPark, Regensburg, Germany

    Susanne Grässel & Sabine Stöckl

  2. Department of Internal Medicine, Experimental Rheumatology and Neuroendocrine—Immunology, University of Regensburg, Regensburg, Germany

    Zsuzsa Jenei-Lanzl

Authors
  1. Susanne Grässel
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  2. Sabine Stöckl
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  3. Zsuzsa Jenei-Lanzl
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Corresponding author

Correspondence to Susanne Grässel .

Editor information

Editors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

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Grässel, S., Stöckl, S., Jenei-Lanzl, Z. (2012). Isolation, Culture, and Osteogenic/Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_14

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  • DOI: https://doi.org/10.1007/978-1-61779-815-3_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

  • eBook Packages: Springer Protocols

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