Abstract
Due to its optical clarity and rudimentary heart structure (i.e., single atrium and ventricle), the zebrafish provides an excellent model for studying the genetic, morphological, and functional basis of normal and pathophysiological heart development in vivo. Recent advances in high-speed confocal imaging have made it possible to capture 2D zebrafish heart wall motions with temporal and spatial resolutions sufficient to characterize the highly dynamic intravital flow-structure environment. We have optimized protocols for introducing fluorescent tracer particles into the zebrafish cardiovasculature, imaging intravital heart wall motion, and performing high-resolution blood flow mapping that will be broadly useful in elucidating flow-structure relationships.
An erratum to this chapter is available at http://dx.doi.org/10.1007/978-1-61779-523-7_28
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-61779-523-7_28
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Acknowledgments
The author would like to thank Steven Gilday and Jian Lu for their work in developing many of the protocols described herein. This work is supported by funding from the National Institutes of Health (R01 RR023190-01).
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Hove, J.R., Craig, M.P. (2012). High-Speed Confocal Imaging of Zebrafish Heart Development. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_26
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DOI: https://doi.org/10.1007/978-1-61779-523-7_26
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