Abstract
(5-N-Succinimidoxy-5-oxopentyl)triphenylphosphonium bromide (SPTPP) is a highly sensitive and positively charged precolumn derivatization reagent for the analysis of amino acids in liquid chromatography–electrospray ionization-tandem mass spectrometry. The synthesis of this reagent and handling of the derivatization reaction are quite simple. It reacts with amino acids rapidly and with high efficiency. MS/MS analysis revealed that the SPTPP-derivatized amino acids formed strong product ions; thus, highly sensitive and selective detection is possible in the selected reaction monitoring mode. The limits of detection for the SPTPP-derivatized amino acids are in the sub-fmol range. The sensitivities of the derivatized amino acids increased about 500-fold, as compared to those of underivatized amino acids.
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Acknowledgments
The authors thank Mr. Yuma Tano and Mr. Yusuke Yamakata for their support with the experiments. This work was supported in part by an academic research grant from the Shimadzu Science Foundation and by the Global COE Program of the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Inagaki, S., Toyo’oka, T. (2012). Amino Acid Analysis via LC–MS Method After Derivatization with Quaternary Phosphonium. In: Alterman, M., Hunziker, P. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 828. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-445-2_5
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DOI: https://doi.org/10.1007/978-1-61779-445-2_5
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