Abstract
Glycan microarray technology enables the screening of large numbers of glycan samples for glycan–protein interactions, based on the presentation of immobilized glycans in a discrete pattern on a solid support. Here we describe a glycan microarray approach employing glycans enzymatically released from proteins and lipids of in vitro cultured cells and of human and animal tissues, followed by the detection of serum antibody binding. This approach may be used to detect autoantibodies in cancer as well as in autoimmune diseases.
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Acknowledgements
The authors thank Dr. C.H. Hokke, Dr. A.R. de Boer, U. Lambertz, Dr. A. van Diepen, and R. Curfs for their help in establishing the array platform. This project was financed by the Netherlands Genomics Initiative (Horizon Breakthrough Project 050-71-302).
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Lonardi, E., Deelder, A.M., Wuhrer, M., Balog, C.I.A. (2012). Microarray Technology Using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection. In: Chevolot, Y. (eds) Carbohydrate Microarrays. Methods in Molecular Biology, vol 808. Humana Press. https://doi.org/10.1007/978-1-61779-373-8_20
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DOI: https://doi.org/10.1007/978-1-61779-373-8_20
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