Abstract
Fluorous-based carbohydrate microarrays provide an alternative to traditional covalent microarray platforms for probing protein–carbohydrate-binding interactions. The most studied plant lectin, concanavalin A (ConA), is known to bind to terminally α-linked mannose. In the studies presented, the binding of ConA with α-mannose is analyzed using a microarray formed on a fluorous-coated glass slide with the sugar containing a fluorous tag at the anomeric position.
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Acknowledgments
We are grateful to Iowa State University, the US National Science Foundation (MCB-0349139 CAREER grant and CHE-0911123) and the US National Institutes of Health (R41 GM075436-01, R42 GM075436, and R42 GM075436-03 S1) with Fluorous Technologies, Inc. for their support of our microarray development work.
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Nagappayya, S.K., Pohl, N.L.B. (2012). Production of Fluorous-Based Microarrays with Uncharged Carbohydrates. In: Chevolot, Y. (eds) Carbohydrate Microarrays. Methods in Molecular Biology, vol 808. Humana Press. https://doi.org/10.1007/978-1-61779-373-8_10
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DOI: https://doi.org/10.1007/978-1-61779-373-8_10
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