Abstract
Stroke is the third leading cause of death and the leading cause of long-term disability in the United States. Brain imaging data from experimental stroke models and stroke patients have shown that there is often a gradual progression of potentially reversible ischemic injury toward infarction. A central core with severely compromised cerebral blood flow (CBF) is surrounded by a rim of moderately ischemic tissue with diminished CBF and impaired electrical activity but preserved cellular metabolism, often referred to as the “ischemic penumbra.” Re-establishing tissue perfusion and/or treating with neuroprotective drugs in a timely fashion is expected to salvage some ischemic tissues. Diffusion-weighted imaging (DWI) based on magnetic resonance imaging (MRI) in which contrast is based on water apparent diffusion coefficient (ADC) can detect ischemic injury within minutes after onsets, whereas computed tomography and other imaging modalities fail to detect stroke injury for at least a few hours. Along with quantitative perfusion imaging, the perfusion–diffusion mismatch which approximates the ischemic penumbra could be defined non-invasively. This chapter describes stroke modeling, perfusion, diffusion, and some other MRI techniques commonly used to image acute stroke and, finally, image analysis pertaining to experimental stroke imaging.
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References
Rosamond, W., Flegal, K., Furie, K. et al. Heart disease and stroke statistics – 2008 update: A report from the American heart association statistics committee and stroke statistics subcommittee. Circulation 2008;117:125–146.
Lloyd-Jones, D., Adams, R., Carnethon, M. et al. Heart disease and stroke statistics – 2009 update: A report from the American heart association statistics committee and stroke statistics subcommittee. Circulation 2009;119:480–486.
Moseley, M. E., Cohen, Y., Mintorovitch, J. et al. Early detection of regional cerebral ischemia in cats: Comparison of diffusion- and T2-weighted MRI and spectroscopy. Magn Reson Med 1990;14:330–346.
Duong, T. Q., Ackerman, J. J. H., Ying, H. S., Neil, J. J. Evaluation of extra- and intracellular apparent diffusion in normal and globally ischemic rat brain via 19F NMR. Magn Reson Med 1998;40:1–13.
Hoehn-Berlage, M., Norris, D. G., Kohno, K., Mies, G., Leibfritz, D., Hossmann, K. -A. Evolution of regional changes in apparent diffusion coefficient during focal ischemia of rat brain: The relationship of quantitative diffusion NMR imaging to reduction in cerebral blood flow and metabolic disturbances. J Cereb Blood Flow Metab 1995;15:1002–1011.
Kohno, K., Hoehn-Berlage, M., Mies, G., Back, T., Hossmann, K. A. Relationship between diffusion-weighted MR images, cerebral blood flow, and energy state in experimental brain infarction. Magn Reson Imag 1995;13:73–80.
Back, M., Hoehn-Berlage, T., PhD, M., Kohno, M. D., Hossmann, K., PhD, M. D., Diffusion Nuclear, K. -A. Magnetic resonance imaging in experimental stroke correlation with cerebral metabolites. Stroke 1994;25:494–500.
Barbier, E. L., Lamalle, L., Decorps, M. Methodology of brain perfusion imaging. J Magn Reson Imaging 2001;13:496–520.
Calamante, F., Thomas, D. L., Pell, G. S., Wiersma, J., Turner, R. Measuring cerebral blood flow using magnetic resonance imaging techniques. J Cereb Blood Flow Metab 1999;19:701–735.
Albers, G. W. Expanding the window for thrombolytic therapy in acute stroke: The potential role of acute MRI for patient selection. Stroke 1999;30:2230–2237.
Heiss, W. D., Graf, R. The ischemic penumbra. Curr Opin Neurol 1994;7:11–19.
NINDS. Tissue plasminogen activator for acute ischemic stroke. The national institute of neurological disorder, and stroke rt-PA stroke study group. N Engl J Med 1995;333:1581–1587.
Rohl, L., Ostergaard, L., Simonsen, C. Z. et al. Viability thresholds of ischemic penumbra of hyperacute stroke defined by perfusion-weighted MRI and apparent diffusion coefficient. Stroke 2001;32:1140–1146.
Schlaug, G., Benfield, A., Baird, A. E. et al. The ischemic penumbra: Operationally defined by diffusion and perfusion MRI. Neurology 1999;53:1528–1537.
Kiozumi, J., Yoshida, Y., Nakazawa, T., Ooneda, G. Experimental studies of ischemic brain edema: I: A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke 1986;8:1–8.
Meng, X., Fisher, M., Shen, Q., Sotak, C. H., Duong, T. Q. Characterizing the diffusion/perfusion mismatch in experimental focal cerebral ischemia. Ann Neurol 2004;55:207–212.
Shen, Q., Meng, X., Fisher, M., Sotak, C. H., Duong, T. Q. Pixel-by-pixel spatiotemporal progression of focal ischemia derived using quantitative perfusion and diffusion imaging. J Cereb Blood Flow Metab 2003;23:1479–1488.
Tatlisumak, T., Carano, R. A. D., Takano, K., Opgenorth, T., Sotak, C. H., Fisher, M. A novel endothelin antagonist, A-127722, attenuates ischemic lesion size in rats with temporal middle cerebral artery occlusion: A diffusion and perfusion MRI study. Stroke 1998;29:850–858.
Macleod, M. R., Fisher, M., O’Collins, V. et al. Good laboratory practice: Preventing introduction of bias at the bench. Stroke 2009;40:e50–e52.
Liu, Z. M., Schmidt, K. F., Sicard, K. M., Duong, T. Q. Imaging oxygen consumption in forepaw somatosensory stimulation in rats under isoflurane anesthesia. Magn Reson Med 2004;52:277–285.
Shen, Q., Ren, H., Cheng, H., Fisher, M., Duong, T. Q. Functional, perfusion and diffusion MRI of acute focal ischemic brain injury. J Cereb Blood Flow Metab 2005;25:1265–1279.
Stejskal, E. O., Tanner, J. E. Spin diffusion measurements: Spin echoes in the presence of a time-dependent field gradient. J Chem Phys 1965;42:288–292.
Silva, A. C., Lee, S. -P., Yang, C., Iadecola, C., Kim, S. -G. Simultaneous blood oxygenation level-dependent and cerebral blood flow functional magnetic resonance imaging during forepaw stimulation in the rat. J Cereb Blood Flow Metab 1999;19:871–879.
Duong, T. Q., Silva, A. C., Lee, S. -P., Kim, S. -G. Functional MRI of calcium-dependent synaptic activity: Cross correlation with CBF and BOLD measurements. Magn Reson Med 2000;43:383–392.
Herscovitch, P., Raichle, M. E. What is the correct value for the brain-blood partition coefficient for water? J Cereb Blood Flow Metab 1985;5:65–69.
Ostergaard, L., Sorensen, A. G., Kwong, K. K., Weisskoff, R. M., Gyldensted, C., Rosen, B. R. High resolution measurement of cerebral blood flow using intravascular tracer bolus passages. Part II: Experimental comparison and preliminary results. Magn Reson Med 1996;36:726–736.
Ostergaard, L., Weisskoff, R. M., Chesler, D. A., Gyldensted, C., Rosen, B. R. High resolution measurement of cerebral blood flow using intravascular tracer bolus passages. Part I: Mathematical approach and statistical analysis. Magn Reson Med 1996;36:715–725.
Reith, W., Hasegawa, Y., Latour, L. L., Dardzinski, B. J., Sotak, C. H., Fisher, M. Multislice diffusion mapping for 3-D evolution of cerebral ischemia in a stroke model. Neurology 1995;45:172–177.
Jacobs, M. A., Zhang, Z. G., Knight, R. A. et al. A Model for multiparametric MRI tissue characterization in experimental cerebral ischemia with histological validation in rat: Part 1. Stroke 2001;32:943–949.
Jacobs, M. A., Knight, R. A., Soltanian-Zadeh, H. et al. Unsupervised segmentation of multiparameter MRI in experimental cerebral ischemia with comparison to T2, diffusion, and ADC MRI parameters and histopathological validation. J Magn Reson Imag 2000;11:425–437.
Shen, Q., Ren, H., Bouley, J., Fisher, M., Duong, T. Q. Dynamic tracking of acute ischemic tissue fates using improved unsupervised ISODATA analysis of high-resolution quantitative perfusion and diffusion data. J Cereb Blood Flow Metab 2004;24:887–897.
Duda, R. O., Hart, P. E. Pattern Classification and Scene Analysis. New York, NY: Wiley; 1973.
Acknowledgments
This work is supported in part by the National Institute of Neurological Disorders and Stroke, the National Institute of Health (R01 NS045879), the Scientist Development Grant (SDG-0430020 N) and the Established Investigator Award (EIA 0940104 N) from the American Heart Association. I thank my former and current colleagues who have participated on these stroke projects.
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Duong, T.Q. (2011). MRI in Experimental Stroke. In: Modo, M., Bulte, J. (eds) Magnetic Resonance Neuroimaging. Methods in Molecular Biology, vol 711. Humana Press. https://doi.org/10.1007/978-1-61737-992-5_24
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DOI: https://doi.org/10.1007/978-1-61737-992-5_24
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