Abstract
Flavonoids are a class of secondary metabolites present in large amounts in grapevine (Vitis vinifera L.), which are involved in several aspects of its physiology (e.g. protection against biotic and abiotic stress). Even if the biosynthetic pathways of flavonoid sub-classes have been largely characterised, the mechanisms of their transport and accumulation to the final target sites are still not completely understood. Unanticipated insights have been obtained by probing plant tissues with pure antibodies targeting bilitranslocase (BTL, TCDB # 2.A.65.1.1), a mammalian transporter involved in the absorption and tissue distribution of dietary flavonoids. The occurrence of a BTL homologue has also been found in grape berries, in both tegumental layers of skin and pulp vascular bundles. In the skin, the expression of this protein starts from véraison (starting of the change in colour and softening of berries) and increases up to a maximum at the harvest stage, matching the same temporal pattern of flavonoid accumulation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Miccio, M., Baldini, G., Basso, V., Gazzin, B., Lunazzi, G. C., Tiribelli, C., and Sottocasa, G. L. (1989) Bilitranslocase is the protein responsible for the electrogenic movement of sulfobromophthalein in plasma membrane vesicles from rat liver: immunochemical evidence using mono- and poly-clonal antibodies. Biochim. Biophys. Acta 981, 115–120.
Passamonti, S., and Sottocasa, G. L. (1990) The sulfhydryl groups responsible for bilitranslocase transport activity respond to the interaction of the carrier with bilirubin and functional analogues. Biochim. Biophys. Acta 1021, 9–12.
Battiston, L., Passamonti, S., Macagno, A., and Sottocasa, G. L. (1998) The bilirubin-binding motif of bilitranslocase and its relation to conserved motifs in ancient biliproteins. Biochem. Biophys. Res. Commun. 247, 687–692.
Passamonti, S., Terdoslavich, M., Margon, A., Cocolo, A., Medic, N., Micali, F., Decorti, G., and Franko, M. (2005) Uptake of bilirubin into HepG2 cells assayed by thermal lens spectroscopy. Function of bilitranslocase. FEBS J. 272, 5522–5535.
Nicolin, V., Grill, V., Micali, F., Narducci, P., and Passamonti, S. (2005) Immunolocalisation of bilitranslocase in mucosecretory and parietal cells of the rat gastric mucosa. J. Mol. Histol. 36, 45–50.
Vanzo, A., Terdoslavich, M., Brandoni, A., Torres, A. M., Vrhovsek, U., and Passamonti. S. (2008) Uptake of grape anthocyanins into the rat kidney and the involvement of bilitranslocase. Mol. Nutr. Food Res. 52, 1106–1116.
Passamonti, S., Vanzo, A., Vrhovsek, U., Terdoslavich, M., Cocolo, A., Decorti, G., and Mattivi, F. (2005) Hepatic uptake of grape anthocyanins and the role of bilitranslocase. Food Res. Int. 38, 953–960.
Passamonti, S., Vrhovsek, U., and Mattivi, F. (2002) The interaction of anthocyanins with bilitranslocase. Biochem. Biophys. Res. Commun. 296, 631–636.
Passamonti, S., Cocolo, A., Braidot, E., Petrussa, E., Peresson, C., Medic, N., Macrì F., and Vianello, A. (2005) Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase. FEBS J. 272, 3282–3296.
Braidot, E., Petrussa, E., Bertolini, A., Peresson, C., Ermacora, P., Loi, N., Terdoslavich, M., Passamonti, S., Macrì, F., and Vianello, A. (2008) Evidence for a putative flavonoid translocator similar to mammalian bilitranslocase in grape berries (Vitis vinifera L.) during ripening. Planta 228, 203–213.
Walker, R. P., Acheson, R. M., Tecsi, L. I., and Leegood, R. C. (1997) Phosphoenolpyruvate carboxykinase in C4 plants: its role and regulation. Aust. J. Plant Physiol. 24, 459–468.
Acknowledgements
Research on this topic was supported by “Tenuta Villanova”, Vineyard and Winery at Farra d’Isonzo (GO), Italy, by Regione Autonoma Friuli-Venezia Giulia (L.R. 26/2005 art. 17) and by Ministero dell’Istruzione, Università e Ricerca (PRIN project 2004070118).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Petrussa, E. et al. (2010). Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries. In: Fett-Neto, A. (eds) Plant Secondary Metabolism Engineering. Methods in Molecular Biology, vol 643. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-723-5_20
Download citation
DOI: https://doi.org/10.1007/978-1-60761-723-5_20
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-722-8
Online ISBN: 978-1-60761-723-5
eBook Packages: Springer Protocols