Skip to main content

In-Situ Hybridization to Sections (Nonradioactive)

  • Protocol
Molecular Embryology

Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 461))

  • 2900 Accesses

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Rex M, Scotting PJ (1994) Simultaneous detection of RNA and protein in tissue sections by nonradioactive in situ hybridization followed by immunohistochemistry. Biochemica [Boehringer Mannheim] 3:24–26.

    Google Scholar 

  2. Angerer L, Angerer R (1991) Localisation of mRNAs by in situ hybridization. Meth Cell Biol 35:37–71.

    Article  CAS  Google Scholar 

  3. Norton AJ, Jordan S, Yeomans P (1994) Brief, high temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J Pathol 173:371–379.

    Article  CAS  PubMed  Google Scholar 

  4. Brouwer N, Van Dijken H, Ruiters MHJ, Van Willigen J-D, Horst GJ (1992) Localisation of dopamine D2 receptor mRNA with non-radioactive in-situ hybridization histochemistry. Neurosci Lett 142:223–227.

    Article  CAS  PubMed  Google Scholar 

  5. Komminoth P, Merk FB, Leav I, Wolfe HJ, Roth J (1992) Comparison of 35S-and digoxigenin-labeled RNA and oligonucleotide probes for ISH. Histochemistry 98217–228.

    Google Scholar 

  6. Rex M, Church R, Tointon K, d Scotting, P. J. (1997) Combination of non-isotopic in situ hybridization with detection of enzyme activity, bromodeoxyuridine incorporation and immunohistochemical markers. Histochem Cell Biol 107:519–523.

    Article  CAS  PubMed  Google Scholar 

  7. Rex M, Uwanogho DA, Orme A, Scotting PJ, Sharpe PT (1997) cSox21 exhibits a complex and dynamic pattern of transcription during embryonic development of the chick central nervous system. Med Dev 66:39–53.

    Article  CAS  Google Scholar 

  8. Rex M, Orme A, Uwanogho D, Tointon K, Wigmore PM, Sharpe PT, Scotting PJ (1997) Dynamic expression of chicken Sox2 and Sox3 genes in ectoderm induced to form neural tissue. Dev Dynamics 209:323–332.

    Article  CAS  Google Scholar 

  9. Uwanogho D, Rex M, Cartwright E, Pearl G, Scotting PJ, Sharpe PT (1995) Embryonic expression of the chicken Sox2, Sox3 and Sox11 genes suggests an interactive role in neuronal development. Mech Dev 49:23–36.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer Science + Business Media, LLC

About this protocol

Cite this protocol

Rex, M., Scotting, P.J. (2008). In-Situ Hybridization to Sections (Nonradioactive). In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. METHODS IN MOLECULAR BIOLOGY™, vol 461. Humana Press. https://doi.org/10.1007/978-1-60327-483-8_49

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-483-8_49

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-431-9

  • Online ISBN: 978-1-60327-483-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics