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The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotox

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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 547))

Summary

Podophyllotoxin is a plant-derived compound found in Podophyllum sp. that is used to produce semi-synthetic anticancer pharmaceuticals such as etoposide, teniposide, and etoposide phosphate. This chapter describes the role of biotechnology to produce podophyllotoxin and our attempts to domesticate Podophyllum peltatum L., also known as the American mayapple. The domestication research on mayapple included surveys of the natural population, identification of high yielding genotypes, propagation, cultivation, sustainable harvest procedures and the development of protocols for in vitro germplasm bank.

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Acknowledgments

This work was partially funded by The University of Mississippi Office of Research and Sponsored Program and by USDA/ARS Specific Cooperative Research Agreement No. 58-6408-2-0009.

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Authors and Affiliations

  1. National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

    Rita M. Moraes

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  1. Hemant Lata
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  2. Cassia S. Mizuno
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  3. Rita M. Moraes
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Correspondence to Rita M. Moraes .

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Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada

    Praveen K. Saxena

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Lata, H., Mizuno, C., Moraes, R. (2009). The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotox. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_31

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  • DOI: https://doi.org/10.1007/978-1-60327-287-2_31

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