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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants pp 359–369Cite as

The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 547))

Summary

Plant cell culture technology is potentially useful in producing high-valued secondary metabolites. Eurycoma longifolia root extracts are consumed as a health tonic but more popularly used as an aphrodisiac. Studies on the aphrodisiac properties and the possible compounds involved have been widely studied. There are many potentially useful compounds reported from the root extracts of E. longifolia. However, studies on the in vitro production of useful compounds from this plant have not been reported. This chapter will describe methods of callus induction and extraction of 9-methoxycanthin-6-one from E. longifolia Jack explants with emphasis on the tap and fibrous roots. This compound, known to have anti-tumour activity, is present in intact plant parts and in callus tissues of different explants.

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Acknowledgments

The 9-methoxycanthin-6-one standard was kindly provided by Professor Chan Kit Lam, School of Pharmaceutical Science, University of Science Malaysia (USM), Penang. This project was supported by the Ministry of Science and Innovation. The authors would like to acknowledge the technical assistance of Mr. M. Hakiman.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia

    Mahmood Maziah

Authors
  1. Mahmood Maziah
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  2. Noormi Rosli
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Corresponding author

Correspondence to Mahmood Maziah .

Editor information

Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada

    Praveen K. Saxena

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Maziah, M., Rosli, N. (2009). The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_29

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  • DOI: https://doi.org/10.1007/978-1-60327-287-2_29

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-286-5

  • Online ISBN: 978-1-60327-287-2

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