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Superoxide Dismutase – A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction

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Free Radicals and Antioxidant Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 610))

Abstract

Erectile dysfunction (ED) is defined as the inability to attain and/or maintain penile erection sufficient for satisfactory sexual performance. Oxidative stress has been demonstrated to be involved in the pathophysiology of age- or diabetes-related ED. Superoxide dismutase (SOD), an antioxidant enzyme catalyzing the conversion of superoxide anion (O2 •–) to hydrogen peroxide (H2O2) and molecular oxygen (O2), is a promising therapeutic target for ED. In vivo gene therapy and adult stem cell-based ex vivo gene therapy are two attractive current gene therapies for the treatment of ED. In this chapter we describe the use of two potent gene transfer techniques to deliver the therapeutic gene extracellular superoxide dismutase (ecSOD) into the penis of aged or diabetic rats for therapy of ED: adenoviral-mediated intracavernosal ecSOD gene transfer for gene therapy of ED and ecSOD gene-modified marrow stromal cells, also known as mesenchymal stem cells, based stem cell and gene therapy.

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Acknowledgments

The authors would like to thank Drs. Beverley Davidson, Yi Chu, and Donald D. Heistad and the University of Iowa Gene Transfer Vector Core for the preparation of adenoviral vectors. Studies presented in this chapter are supported in part by NIH grant HL62000.

Author information

Authors and Affiliations

  1. Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, LA, USA

    W. Deng, T.J. Bivalacqua & Philip J. Kadowitz

  2. Department of Urology, Johns Hopkins Hospital, Baltimore, MD, USA

    T.J. Bivalacqua

  3. Department of Medicine University, Pittsburg, PA, USA

    H.C. Champion

  4. Department of Urology, Tulane University Health Sciences Center, New Orleans, LA, USA

    W.J. Hellstrom

  5. Departments of Medicine and Pharmacology, Tulane University School of Medicine, New Orleans, LA, USA

    Subramanyam N. Murthy

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  1. W. Deng
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  2. T.J. Bivalacqua
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  5. Subramanyam N. Murthy
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  6. Philip J. Kadowitz
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Editor information

Editors and Affiliations

  1. A& M College, Southern University &, James L. Hunt Street, Baton Rouge, 70813, U.S.A.

    Rao M. Uppu

  2. School of Medicine, Tulane University, Tulane Ave. 1430, New Orleans, 70112, U.S.A.

    Subramanyam N. Murthy

  3. Biodynamics Inst., Louisiana State University, Baton Rouge, 70808, U.S.A.

    William A. Pryor

  4. Dorothy M. Davis Heart &, Ohio State University, West 12th Ave. 473, Columbus, 43210, U.S.A.

    Narasimham L. Parinandi

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Deng, W., Bivalacqua, T., Champion, H., Hellstrom, W., Murthy, S.N., Kadowitz, P.J. (2010). Superoxide Dismutase – A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction. In: Uppu, R., Murthy, S., Pryor, W., Parinandi, N. (eds) Free Radicals and Antioxidant Protocols. Methods in Molecular Biology, vol 610. Humana Press. https://doi.org/10.1007/978-1-60327-029-8_13

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  • DOI: https://doi.org/10.1007/978-1-60327-029-8_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-710-5

  • Online ISBN: 978-1-60327-029-8

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