Abstract
Technological advances in the acquisition of DNA and protein sequence information and the resulting onrush of data can quickly overwhelm the scientist unprepared for the volume of information that must be evaluated and carefully dissected to discover its significance. Few laboratories have the luxury of dedicated personnel to organize, analyze, or consistently record a mix of arriving sequence data. A methodology based on a modern relational-database manager is presented that is both a natural storage vessel for antibody sequence information and a conduit for organizing and exploring sequence data and accompanying annotation text. The expertise necessary to implement such a plan is equal to that required by electronic word processors or spreadsheet applications. Antibody sequence projects maintained as independent databases are selectively unified by the relational-database manager into larger database families that contribute to local analyses, reports, interactive HTML pages, or exported to facilities dedicated to sophisticated sequence analysis techniques. Database files are transposable among current versions of Microsoft, Macintosh, and UNIX operating systems.
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References
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Acknowledgments
The author would like to thank the following members of the Protein Interactions Group, Center for Cancer Research Nanobiology Program, for sequence contributions, support, and invaluable suggestions for improvements and additional features: Samitabh Chakraborti, Weizao Chen, Vidita Choudhry, Dimiter Dimitrov, Yang Feng, Igor Sidorov, Xiaodong Xiao, Mei-Yun Zhang, Zhongyu Zhu. This project was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Owens, J. (2009). Organizing, Exploring, and Analyzing Antibody Sequence Data: The Case for Relational-Database Managers. In: Dimitrov, A. (eds) Therapeutic Antibodies. Methods in Molecular Biology™, vol 525. Humana Press. https://doi.org/10.1007/978-1-59745-554-1_32
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DOI: https://doi.org/10.1007/978-1-59745-554-1_32
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Publisher Name: Humana Press
Print ISBN: 978-1-934115-92-3
Online ISBN: 978-1-59745-554-1
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