Abstract
ImmunoRNases represent a highly attractive alternative to conventional immunotoxins for cancer therapy. Quantitative production of immunoRNases in appropriate expression systems, however, remains a major challenge for further clinical development of these novel compounds. Here we describe a method for high-level production and purification of a fully functional immunoRNase fusion protein from supernatants of stably transfected mammalian cells.
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Krauss, J., Exner, E., Mavratzas, A., Seeber, S., Arndt, M.A. (2009). High-Level Production of a Humanized ImmunoRNase Fusion Protein from Stably Transfected Myeloma Cells. In: Dimitrov, A. (eds) Therapeutic Antibodies. Methods in Molecular Biology™, vol 525. Humana Press. https://doi.org/10.1007/978-1-59745-554-1_24
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DOI: https://doi.org/10.1007/978-1-59745-554-1_24
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