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Protein Blotting and Detection pp 417–432Cite as

Whole and Strip Nitrocellulose Membrane as well as a New-Line-Immunoblotting ofAntigen Using the Chemiluminescence Technique

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 536))

Summary

There are a number of techniques in the scientific world that researchers use to detect specific antigens. One such technique that has provided many advantages over typical immunochemical staining is chemiluminescence. The emission of visible radiation by compounds once exposed to sunlight has been known for centuries and currently is the main principle for chemiluminescence. Here, we introduce three different chemiluminescence techniques that are widely used in immunodetection of antigens: (a) whole membrane chemiluminescence detection, (b) strip membrane chemiluminescence detection, and (c) new line blotting chemiluminescence.

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References

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Author information

Authors and Affiliations

  1. Oklahoma Medical Research Foundation, 825 NE 13th St, Arthritis and Immunology Department, MS #24, Oklahoma City, OK, 73104, USA

    Yaser Dorri, Ali W. Khalili & R. Hal Scofield

Authors
  1. Yaser Dorri
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  2. Ali W. Khalili
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  3. R. Hal Scofield
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Corresponding author

Correspondence to Yaser Dorri .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    R. Hal Scofield

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Dorri, Y., Khalili, A.W., Scofield, R.H. (2009). Whole and Strip Nitrocellulose Membrane as well as a New-Line-Immunoblotting ofAntigen Using the Chemiluminescence Technique. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_42

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  • DOI: https://doi.org/10.1007/978-1-59745-542-8_42

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-73-2

  • Online ISBN: 978-1-59745-542-8

  • eBook Packages: Springer Protocols

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