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A miniaturized blotting system for simultaneous detecting of differentautoantibodies

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Protein Blotting and Detection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 536))

Summary

Sera of tumor patients frequently contain autoantibodies to tumor-associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.

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Acknowledgements

This work was supported in part by grants from the Medical Faculty of the TU-Dresden and the BMBF (Germany). We like to thank Martina Franke for her excellent technical support.

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Authors and Affiliations

  1. Institut für Immunologie, Carl Gustav Carus Technische Universität Dresden, Fetscherstrasse 74, 01307, Dresden, Germany

    Ulrich Canzler, Holger Bartsch, Kai Großmann, Werner Lehmann, Karsten Conrad, Biji T. Kurien, Yaser Dorri, R. Hal Scofield & Michael Bachmann

Authors
  1. Ulrich Canzler
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  2. Holger Bartsch
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  3. Kai Großmann
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  4. Werner Lehmann
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  5. Karsten Conrad
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  6. Biji T. Kurien
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  7. Yaser Dorri
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  8. R. Hal Scofield
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  9. Michael Bachmann
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Corresponding author

Correspondence to Michael Bachmann .

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Editors and Affiliations

  1. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    R. Hal Scofield

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Canzler, U. et al. (2009). A miniaturized blotting system for simultaneous detecting of differentautoantibodies. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_15

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  • DOI: https://doi.org/10.1007/978-1-59745-542-8_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-73-2

  • Online ISBN: 978-1-59745-542-8

  • eBook Packages: Springer Protocols

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