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Proteolytic Profiling of the Extracellular Matrix Degradome

  • Protocol
Peptide Characterization and Application Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 386))

Summary

The profiling of protein function is one of the most challenging scientific tasks in the postgenomic age. Traditional protein expression methodologies have focused only on the quantification of proteins under varying conditions or pathologies. Determining the functional differences between protein populations allows for a more accurate view of the outcomes in normal vs diseased proteomes. Because the presence or absence of a protein’s function can affect its complex surroundings (consisting of multiple other proteins and substrates), the study of proteome functionality yields information on protein-protein interactions, amplification cascades, signaling pathways, and posttranslational modifications. Of significant interest are proteinases, as proteolysis is responsible for tight regulation of various cellular and tissue processes. Proteinase activities, or lack there of, alter the proteome makeup by regulating other proteins or by generating cleavage products. This chapter describes current proteolytic profiling technologies using activity or target-based formats. In particular, the analysis of collagenolytic matrix metalloproteinase activity using fluorogenic triple-helical substrates is discussed.

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Acknowledgments

We gratefully acknowledge the support of the National Institutes of Health CA77402 and CA98799 (to G.B.F.) and the FAU Center of Excellence in Biomedical and Marine Biotechnology (contribution #P200506).

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Authors and Affiliations

  1. Department of Chemistry & Biochemistry, Florida Atlantic University, Boca Raton, FL

    Diane Baronas-Lowell & Mohammad Al-Ghoul

  2. College of Biomedical Sciences and Department of Chemistry & Biochemistry, Florida Atlantic University, Boca Raton, FL

    Janelle L. Lauer-Fields & Gregg B. Fields

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  1. Diane Baronas-Lowell
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  2. Janelle L. Lauer-Fields
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  3. Mohammad Al-Ghoul
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  4. Gregg B. Fields
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Editors and Affiliations

  1. Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton, FL

    Gregg B. Fields

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Baronas-Lowell, D., Lauer-Fields, J.L., Al-Ghoul, M., Fields, G.B. (2007). Proteolytic Profiling of the Extracellular Matrix Degradome. In: Fields, G.B. (eds) Peptide Characterization and Application Protocols. Methods in Molecular Biology™, vol 386. Humana Press. https://doi.org/10.1007/978-1-59745-430-8_6

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  • DOI: https://doi.org/10.1007/978-1-59745-430-8_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-550-7

  • Online ISBN: 978-1-59745-430-8

  • eBook Packages: Springer Protocols

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