Abstract
In vivo preclinical assays are required to screen potential agents that target the tumor vasculature. Here, a hollow fibre-based assay for the quantification of neovasculature in the presence or absence of an agent that potentially targets tumor neovasculature is described. The neovasculature is developed as a consequence of the presence of tumor cells encapsulated in hollow fibres, which are transplanted subcutaneously in the dorsal flanks of mice.
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Acknowledgements
I would like to thank Tricia Cooper for her advice and practical input and Shofiq Al-Islam for his additional technical assistance. This work was supported by Cancer Research UK Programme grant C7589/A5953.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Shnyder, S.D. (2009). Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature. In: Murray, C., Martin, S. (eds) Angiogenesis Protocols. Methods in Molecular Biology, vol 467. Humana Press. https://doi.org/10.1007/978-1-59745-241-0_21
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DOI: https://doi.org/10.1007/978-1-59745-241-0_21
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