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Pharmacogenetics: Role of Single Nucleotide Polymorphisms

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

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Abstract

Genome sequencing methods have basically similar algorithms, although they show a few differences between the platforms. The human genome contains approximately three billion base pairs, and this amount is huge and therefore impossible to sequence at one step. However, this amount is not a problem for developed technology. Researchers break DNA into small random pieces and then sequence and reassemble. Library preparation, sequencing, bioinformatic approaches and reporting. High-quality library preparation is critical and the most important part of the next-generation sequencing workflow. Successful sequencing directly requires high-quality libraries. Sequencing is second step and all high-throughput sequencing approaches are generally based on conventional Sanger sequencing. After preparation of library and sequencing, later steps are completely computer-based (in silico) approaches called as bioinformatics.

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Author information

Authors and Affiliations

  1. Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul, Turkey

    Emrah Yucesan & Nur Ozten

  2. Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Bezmialem Vakif University, Istanbul, Turkey

    Nur Ozten

Authors
  1. Emrah Yucesan
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  2. Nur Ozten
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Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Yucesan, E., Ozten, N. (2019). Pharmacogenetics: Role of Single Nucleotide Polymorphisms. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

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