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Fluorescence In Situ Hybridization and Rehybridization Using Bacterial Artificial Chromosome Probes

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

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Abstract

Fluorescence in situ hybridization (FISH) method enables in situ genetic analysis of both metaphase and interphase cells from different types of material, including cell lines, cell smears, and fresh and paraffin-embedded tissue. Despite the growing number of commercially available FISH probes, still for large number of gene loci or chromosomal regions commercial probes are not available. Here we describe a simple method for generating FISH probes using bacterial artificial chromosomes (BAC). Due to genome-wide coverage of BAC clones, there are almost unlimited possibilities for the analysis of any genomic regions using BAC FISH probes.

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Authors and Affiliations

  1. Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, London, UK

    Elzbieta Stankiewicz, Tianyu Guo, Xueying Mao & Yong-Jie Lu

Authors
  1. Elzbieta Stankiewicz
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  2. Tianyu Guo
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  3. Xueying Mao
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  4. Yong-Jie Lu
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Corresponding author

Correspondence to Yong-Jie Lu .

Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Stankiewicz, E., Guo, T., Mao, X., Lu, YJ. (2019). Fluorescence In Situ Hybridization and Rehybridization Using Bacterial Artificial Chromosome Probes. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

  • eBook Packages: Springer Protocols

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