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Aptamer Selection for Detecting Molecular Target Using Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Technology

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

Abstract

Cell-SELEX is a live cell-based in vitro selection method that generates functional oligonucleotides, or aptamers. Often referenced as the chemist’s antibody, aptamers bind to select targets with high affinity and can be utilized in a number of applications, including biomedicine, bioimaging, and biosensing. Here we describe the cell-SELEX technique and discuss this methodology’s unique merit(s)—namely the ability to isolate highly selective aptamer panels with no prior knowledge of cellular signatures. This strategy thus presents as a technology that has the potential to enhance the precision of molecular medicine and targeted therapeutics.

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Authors and Affiliations

  1. Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute, McKnight Brain Institute, University of Florida, Gainesville, FL, USA

    Kimberly D. Stewart & Weihong Tan

  2. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, People’s Republic of China

    Weihong Tan

  3. Department of Cancer Epidemiology, Moffitt Cancer Center, Tampa, FL, USA

    Jong Y. Park

Authors
  1. Kimberly D. Stewart
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  2. Weihong Tan
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  3. Jong Y. Park
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Corresponding author

Correspondence to Jong Y. Park .

Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Stewart, K.D., Tan, W., Park, J.Y. (2019). Aptamer Selection for Detecting Molecular Target Using Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Technology. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_15

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

  • eBook Packages: Springer Protocols

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