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Yeast Genome Screening and Methods for the Discovery of Metabolic Pathways Involved in a Phenotypic Response to Anticancer Agents

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Yeast Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2049))

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Abstract

The dramatic increase of cancer in the world drives the search for a new generation of drugs useful in effective and safe chemotherapy. In the postgenomic era the use of the yeast Saccharomyces cerevisiae as a simple eukaryotic model is required in molecular studies of biological activity of compounds that may be potential drugs in the future. The phenotype analysis of numerous deletion mutants (from the EUROSCARF collection) allows one to define the specific influence of tested compound on metabolism, stress generation and response of eukaryotic cell to stress. Moreover, it allows one to determine cell viability, design of new drugs and doses used in preclinical and clinical trials. Undoubtedly, this is also a good way to save the lives of many laboratory animals. Here we present a simple and cheap new approach to study the metabolic and stress response pathways in eukaryotic cells involved in the response to tested compounds (e.g., anticancer agents). The precise determination of biological activity mechanisms of tested compounds at the molecular level can contribute to the fast introduction of new cancer therapies, which is extremely important nowadays.

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Author information

Authors and Affiliations

  1. Department of Genetics, Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw, Poland

    Magdalena Cal, Irwin Matyjaszczyk & Stanisław Ułaszewski

Authors
  1. Magdalena Cal
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  2. Irwin Matyjaszczyk
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  3. Stanisław Ułaszewski
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Corresponding author

Correspondence to Stanisław Ułaszewski .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, Cambridge, UK

    Stephen G. Oliver

  2. Genetadi Biotech, Parque Tecnológico de Bizkaia, Biscay, Spain

    Juan I. Castrillo

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Cal, M., Matyjaszczyk, I., Ułaszewski, S. (2019). Yeast Genome Screening and Methods for the Discovery of Metabolic Pathways Involved in a Phenotypic Response to Anticancer Agents. In: Oliver, S.G., Castrillo, J.I. (eds) Yeast Systems Biology. Methods in Molecular Biology, vol 2049. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9736-7_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9736-7_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9735-0

  • Online ISBN: 978-1-4939-9736-7

  • eBook Packages: Springer Protocols

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