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G-Quadruplex Nucleic Acids pp 407–433Cite as

19F NMR Spectroscopy for the Analysis of DNA G-Quadruplex Structures Using 19F-Labeled Nucleobase

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2035))

Abstract

G-quadruplex structures have been suggested to be biologically important in processes such as transcription and translation, gene expression and regulation in human cancer cells, and regulation of telomere length. Investigation of G-quadruplex structures associated with biological events is therefore essential to understanding the functions of these molecules. We developed the 19F-labeled nucleobases and introduced them into DNA sequences for the 19F NMR spectroscopy analysis. We present the 19F NMR methodology used in our research group for the study of G-quadruplex structures in vitro and in living cells.

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Acknowledgments

This work is supported by JSPS KAKENHI (26288083, 17H03091, 16K17938). Support from the Takeda Science Foundation and Nakatani Foundation Scholarship is also acknowledged.

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Authors and Affiliations

  1. Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

    Takumi Ishizuka, Hong-Liang Bao & Yan Xu

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  1. Takumi Ishizuka
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  2. Hong-Liang Bao
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  3. Yan Xu
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Correspondence to Yan Xu .

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Editors and Affiliations

  1. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Purdue Center for Cancer Research, Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

  2. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin

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Ishizuka, T., Bao, HL., Xu, Y. (2019). 19F NMR Spectroscopy for the Analysis of DNA G-Quadruplex Structures Using 19F-Labeled Nucleobase. In: Yang, D., Lin, C. (eds) G-Quadruplex Nucleic Acids. Methods in Molecular Biology, vol 2035. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9666-7_26

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  • DOI: https://doi.org/10.1007/978-1-4939-9666-7_26

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9665-0

  • Online ISBN: 978-1-4939-9666-7

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