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G-Quadruplex Nucleic Acids pp 333–345Cite as

Targeting G-Quadruplexes with PNA Oligomers

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2035))

Abstract

The growing interest in G-quadruplex (G4) structure and function is motivating intense efforts to develop G4-binding ligands. This chapter describes the design and testing of peptide nucleic acid (PNA) oligomers, which can bind to G4 DNA or RNA in two distinct ways, leading to formation of heteroduplexes or heteroquadruplexes. Guidelines for designing G4-targeting PNAs and step-by-step protocols for characterizing their binding through biophysical or biochemical methods are provided.

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Acknowledgments

Our work in this area has been supported by the National Institutes of Health (R01 GM58547) and the David Scaife Family Charitable Foundation (141RA01).

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Authors and Affiliations

  1. Department of Chemistry and Center for Nucleic Acids Science and Technology, Carnegie Mellon University, Pittsburgh, PA, USA

    Bruce A. Armitage

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  1. Bruce A. Armitage
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Correspondence to Bruce A. Armitage .

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Editors and Affiliations

  1. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Purdue Center for Cancer Research, Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

  2. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin

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Armitage, B.A. (2019). Targeting G-Quadruplexes with PNA Oligomers. In: Yang, D., Lin, C. (eds) G-Quadruplex Nucleic Acids. Methods in Molecular Biology, vol 2035. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9666-7_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9666-7_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9665-0

  • Online ISBN: 978-1-4939-9666-7

  • eBook Packages: Springer Protocols

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