Abstract
Dendritic cells (DCs) are powerful antigen presenting cells that are involved in regulating immune response. Plasmacytoid dendritic cells (pDCs) are subtype of DCs that present in small quantity in the bone marrow, peripheral blood, and lymph nodes. They are important component of our immune system in normal condition and diseases. They activate T cells and play a critical role in immune tolerance. In this chapter we review the immunophenotypic features of pDCs and provide a practical protocol for pDCs enumeration in the peripheral blood and bone marrow samples.
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References
Reddy V, Iturraspe JA, Tzolas AC (2004) Low dendritic cell count after allogeneic hematopoietic stem cell transplantation predicts relapse, death, and acute graft-versus-host disease. Blood 103(11):4330–4335. Epub 2004 Feb 12
Abe M, Wang Z, de Creus A (2005) Plasmacytoid dendritic cell precursors induce allogeneic T-cell hyporesponsiveness and prolong heart graft survival. Am J Transplant 5(8):1808–1819
McKenna K, Beignon AS, Bhardwaj N (2005) Plasmacytoid dendritic cells: linking innate and adaptive immunity. J Virol 79(1):17–27. Review
Tomasello E, Naciri K, Chelbi R (2018) Molecular dissection of plasmacytoid dendritic cell activation in vivo during a viral infection. EMBO J 37(19):e98836
Auletta JJ, Devine SM, Waller EK (2016) Plasmacytoid dendritic cells in allogeneic hematopoietic cell transplantation: benefit or burden? Bone Marrow Transplant 51(3):333–343
Swiecki M, Colonna M (2015) The multifaceted biology of plasmacytoid dendritic cells. Nat Rev Immunol 15(8):471–485
Elze MC, Ciocarlie O, Heinze A (2015) Dendritic cell reconstitution is associated with relapse-free survival and acute GVHD severity in children after allogeneic stem cell transplantation. Bone Marrow Transplant 50(2):266–273
Su RJ, Green R, Chen M (2018) Enumeration of bone marrow plasmacytoid dendritic cells by multiparameter flow cytometry as a prognostic marker following allogeneic hematopoietic stem cell transplantation. Blood Cells Mol Dis 69:107–112
Pilley J, Chen W, Fuda F et al (2017) The immunophenotypic appearance of plasmacytoid dendritic cells in various specimens. Mod Pathol 30(Suppl):370A
Swerdlow SH, Campo E, Jaffe ES et al (2017) WHO classification of tumours of haematopoietic and lymphoid tissues, 4th edn. IARC Press, Lyon, pp 174–177
Chen W, Luu HS (2017) Immunophenotyping by multiparameter flow cytometry. Methods Mol Biol 1633:51–73
Alculumbre S, Pattarini L (2016) Purification of human dendritic cell subsets from peripheral blood. Methods Mol Biol 1423:153–167
Setoodeh S, Fuda F, Chen W et al (2017) Flow cytometric immunophenotypic features of blastic plasmacytoid dendritic cell neoplasm: a single institution experience. Mod Pathol 30(Suppl):376A–377A
MartÃn-MartÃn L, Almeida J, Hernández-Campo PM (2009) Immunophenotypical, morphologic, and functional characterization of maturation-associated plasmacytoid dendritic cell subsets in normal adult human bone marrow. Transfusion 49(8):1692–1708
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Alsuwaidan, A., Fuda, F., Chen, W., Chen, M. (2019). Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric Analysis. In: McCoy, Jr, J. (eds) Immunophenotyping. Methods in Molecular Biology, vol 2032. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9650-6_11
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DOI: https://doi.org/10.1007/978-1-4939-9650-6_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9649-0
Online ISBN: 978-1-4939-9650-6
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