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Skin and Soft Tissue Models for Acinetobacter baumannii Infection

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Acinetobacter baumannii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

Multidrug-resistant A. baumannii are important Gram-negative pathogens causing persistent wound infections in both wounded and burned victims, which often result in secondary complications such as delayed wound healing, skin graft failure, and sometimes more serious outcomes such as sepsis and amputation. The choice of antibiotics to remediate these A. baumannii infections is becoming limited; and therefore, there has been a renewed interest in the research and development of new antibacterials targeting this pathogen. However, the evaluation of safety and efficacy is made more difficult by the lack of well-established in vivo models. This chapter describes established rodent and large animal models that have been used to investigate and develop treatments for A. baumannii skin and soft tissue infections.

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Acknowledgments

Our research has been supported by a number of grants from the Military Infectious Diseases Research and Development Program as well as supplemental funding from the Defense Health Agency. We would like to thank all the members of the Wound Infections Department, past and present, who have contributed to this work. We would also like to thank MAJ Matt Johnson of the NMRC and the Navy Dive team who supplied the custom vest for the pigs.

Material has been reviewed by the Walter Reed Army Institute of Research. There is no objection to its presentation and/or publication. The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting true views of the Department of the Army or the Department of Defense. Research was conducted under approved animal protocols in an AAALAC-accredited facility in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, NRC publication, 2011 edition.

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Authors and Affiliations

  1. Wound Infections Department, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA

    Daniel V. Zurawski, Jaideep Banerjee, Yonas A. Alamneh, Jonathan P. Shearer & Samandra T. Demons

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  1. Daniel V. Zurawski
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  2. Jaideep Banerjee
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  3. Yonas A. Alamneh
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  4. Jonathan P. Shearer
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  5. Samandra T. Demons
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Corresponding author

Correspondence to Daniel V. Zurawski .

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Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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Zurawski, D.V., Banerjee, J., Alamneh, Y.A., Shearer, J.P., Demons, S.T. (2019). Skin and Soft Tissue Models for Acinetobacter baumannii Infection. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_25

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

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