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Desiccation Tolerance Assays for Acinetobacter baumannii

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Acinetobacter baumannii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of A. baumannii in clinical settings through contaminated healthcare equipment and personnel. Desiccation is a multifaceted stress, and many physiological and environmental factors can influence its impact on bacterial survival. This chapter provides a protocol for assessing desiccation survival that facilitates comparisons among A. baumannii strains under various environmental conditions.

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Acknowledgment

This work was supported by ARO grant W911NF-16-1-0146 to B.W.D., NIH grant AI125337 to B.W.D, and NIH grants AI064184, AI76322, AI119879, and AI129940 to M.S.T.

Author information

Authors and Affiliations

  1. Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA

    Xun Wang & Bryan W. Davies

  2. Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, GA, USA

    M. Stephen Trent

  3. Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA

    Bryan W. Davies

  4. Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA

    Bryan W. Davies

  5. John Ring LaMontagne Center for Infectious Diseases, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA

    Bryan W. Davies

Authors
  1. Xun Wang
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  2. M. Stephen Trent
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  3. Bryan W. Davies
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Corresponding author

Correspondence to Bryan W. Davies .

Editor information

Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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Wang, X., Trent, M.S., Davies, B.W. (2019). Desiccation Tolerance Assays for Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

  • eBook Packages: Springer Protocols

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