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Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain

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Astrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1938))

Abstract

Double immunohistochemistry and confocal microscopy permits to detect the coverage of blood vessels by astrocytic endfeet in human postmortem brain tissue. Here we describe double immunofluorescent staining for detecting a colocalization of a marker of astrocytic processes (labeled with an antibody for aquaporin-4 (AQP4) and a marker for blood vessels (labeled with an antibody to collagen IV). Then, we present a microscopic analysis of the coverage of blood vessels by astrocytic processes using Nikon C1 confocal microscope, Photoshop, and ImageJ software.

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Authors and Affiliations

  1. Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson, MS, USA

    Grazyna Rajkowska & Jose Javier Miguel-Hidalgo

Authors
  1. Grazyna Rajkowska
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  2. Jose Javier Miguel-Hidalgo
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Corresponding author

Correspondence to Grazyna Rajkowska .

Editor information

Editors and Affiliations

  1. Department of Psychiatry and Psychotherapy, University of Regensburg, Regensburg, Germany

    Barbara Di Benedetto

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Rajkowska, G., Miguel-Hidalgo, J.J. (2019). Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain. In: Di Benedetto, B. (eds) Astrocytes. Methods in Molecular Biology, vol 1938. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9068-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9068-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9067-2

  • Online ISBN: 978-1-4939-9068-9

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